Nuclear Factor-1 and Metal Transcription Factor-1 Synergistically Activate the Mouse Metallothionein-1 Gene in Response to Metal Ions

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Nuclear factor-1 and metal transcription factor-1 synergistically activate the mouse metallothionein-1 gene in response to metal ions.

Metal activation of metallothionein (MT) gene transcription is dependent on the presence of metal regulatory elements (MREs), which are present in five non-identical copies (MREa through MREe) in the promoter of the mouse MT-1 gene and on the capacity of metal transcription factor-1 (MTF-1) to bind to the MREs in the presence of zinc. We detected a protein, distinct from MTF-1, specifically bin...

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1 Nuclear Factor 1 and Metal Transcription Factor - 1 Synergistically Activate the Mouse Metallothionein - 1 Gene in Response to Metal Ions

NUCLEAR FACTOR 1 AND METAL TRANSCRIPTION FACTOR-1 SYNERGISTICALLY ACTIVATE THE MOUSE METALLOTHIONEIN-1 GENE IN RESPONSE TO METAL IONS* Olivier LaRochelle, Simon Labbé, Jean-François Harrisson, Carl Simard, Véronique Tremblay, Geneviève St-Gelais & Carl Séguin From Centre de recherche en cancérologie de l’Université Laval, CHUQ, Hôtel-Dieu de Québec, Québec (Québec) Canada G1R 2J6, and Départeme...

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Activation of metallothionein gene expression by hypoxia involves metal response elements and metal transcription factor-1.

Metallothioneins (MTs) are a family of stress-induced proteins with diverse physiological functions, including protection against metal toxicity and oxidants. They may also contribute to the regulation of cellular proliferation, apoptosis, and malignant progression. We reported previously that the human (h)MT-IIA isoform is induced in carcinoma cells (A431, SiHa, and HT29) exposed to low oxygen...

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Molecular Mechanism of the Metallothionein Gene Expression Mediated by Metal-Responsive Transcription Factor 1

Metal-responsive transcription factor 1 (MTF-1) is essential for activating transcription of the metallothionein genes in response to multiple species of heavy metals by binding to the metal-responsive element (MRE) located in the upstream region of the genes. In contrast, MTF-1 binding to the MRE in vitro is induced only by zinc ions, suggesting that MTF-1 acts as a zinc sensor protein. Althou...

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Balance between metallothionein and metal response element binding transcription factor 1 is mediated by zinc ions (review).

Metal ion homeostasis and heavy metal detoxification systems are regulated by certain genes associated with metal ion transport. Metallothionein (MT) and metal response element binding transcription factor 1 (MTF‑1) are important regulatory proteins involved in the mediation of intracellular metal ion balance. Differences in the zinc‑binding affinities of the zinc fingers of MTF‑1 and the α‑ an...

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ژورنال

عنوان ژورنال: Journal of Biological Chemistry

سال: 2008

ISSN: 0021-9258

DOI: 10.1074/jbc.m800640200